Monday noon 07 June 2010: Session I

Author	Title	Year	Company (Country)	Reftype
Büchs, J.	The RoboLector, a sytem for automated microtiter plate cultivations with full on-line measuring capability [Abstract]	2010	RWTH Aachen University (Germany)	standard
Abstract: Although modern methods of molecular biology and systems biology has led to a rational design of mammalian cells, screening of large numbers of clones and media is still one of the most important tasks in biotechnology. For this purpose shaken flasks and increasingly microtiter plates are employed. However, suitable, defined and reproducible experimental conditions are required to obtain meaningful results. Therefore, the culture conditions must be carefully evaluated and characterised. Mixing problems are easily encountered especially in microtiter plates and are not always detected due to absence of on-line monitoring. An insufficient treatment and nutrient supply of the cells can alter metabolism and hence mislead the outcome of the experiment. It is also a very common practice in large screening projects that only the final product titre is measured at the end of the culture for the evaluation of the “best performes”. In the recent years several approaches were introduced to follow microbial cultures in shaken bioreactors like shake flasks or microtiter plates. It became obvious that the cultures can behave in a quite unexpected way and most relevant and essential information is lost, if only the final product titer at the end of the cultures is utilised for evaluation. As a result, the screening may be directed to an unknown and non-intended direction or may even fail. New methods and techniques are introduced to measure the oxygen and carbon dioxide transfer rate and the respiratory quotient in shake flasks and microtiter plates (RAMOS) and the optical density, NADH fluorescence, pH and DOT by optical means in microtiter plates (BioLector). If the desired product can be fused to a fluorescence protein, like GFP or YFP, also the product formation can be monitored on-line. It is of utmost importance that the operating conditions of the applied shaking bioreactors are suitable and shaking motion is not stopped during the measurement. Otherwise e.g. power input, mixing and oxygen supply is interrupted and the micro-organisms will ongoingly rearrange their metabolism to cope with these disturbances of their environmental conditions. Therefore, our on-line monitoring techniques are applicable during continuous shaking of the bioreactors. The RoboLector is the newest device in this family. It is comprised of a combination of the BioLector and a pipetting robot with a Hepa filter (laminar flow bench). This makes completely automated experimentation possible. This will be demonstrated by an inductor profiling. Inductor concentration and induction time is varied in the same experiment. The data of a single experiment will provide detailed information on the optimal induction conditions. Results obtained for CHO and insect cells will be reported.
BibTeX: @standard{Buechs2010, author = {Jochen Büchs}, title = {The RoboLector, a sytem for automated microtiter plate cultivations with full on-line measuring capability}, journal = {RWTH Aachen University (Germany)}, year = {2010} }
Giroux, D. & Yunghans*, A.	Novel single-use bioreactors using a pneumatic mixing mechanism: Case studies of large scale evaluation (250 L) and scale-down model (2 L) development [Abstract]	2010	PBS Biotech, Inc., *Amgen Bioprocessing Center (USA)	standard
Abstract: A novel single-use bioreactor system has been developed by utilizing a pneumatic mixing mechanism which converts the buoyancy of inlet gas bubbles to a rotational energy by an air-wheelTM. Since this technology does not require any external mechanical device for liquid agitation, it can offer a simple, compact, and scalable bioreactor system. Cell culture performance in this pneumatic bioreactor system (PBS) was studied and compared to that of other bioreactor systems including the conventional stirred bioreactors and wave-type bioreactors. In addition, scalability of the system was evaluated for biological cell culture performance as well as for liquid mixing performance via computational fluid dynamics (CFD’s). The performance of the small scale unit was characterized as a representative scale-down model that can be used for process development. In this presentation, we will introduce the new features of this novel disposable bioreactor and then discuss the results from biological tests involving CHO cell lines expressing recombinant monoclonal antibodies.
BibTeX: @standard{Giroux2010, author = {Daniel Giroux and Andrew Yunghans*}, title = {Novel single-use bioreactors using a pneumatic mixing mechanism: Case studies of large scale evaluation (250 L) and scale-down model (2 L) development}, journal = {PBS Biotech, Inc., **Amgen Bioprocessing Center (USA)}, year = {2010} }
Hodge, G.	Production of viral, Virus Like Particle (VLP) and soluble subunit vaccines in mammalian and microbial single-use stirred tank bioreactors [Abstract]	2010	Xcellerex Inc. (USA)	standard
Abstract: Three different types of vaccines have been produced in mammalian single-use stirred tank bioreactors: a virus based vaccine produced by Vero cells on microcarriers, soluble West Nile subunit vaccine produced by S2 insect cells and virus like particles (VLP) produced by SF9 insect cells. In addition, three different antigens have been produced in microbial single-use bioreactors: recombinant protective antigen subunit vaccine, swine flu hemagglutinin (H1, California strain) and HIV Nef antigen. The paper will present kinetics of cell growth and product formation in mammalian bioreactors and microbial fermenters.
BibTeX: @standard{Hodge2010, author = {Geoff Hodge}, title = {Production of viral, Virus Like Particle (VLP) and soluble subunit vaccines in mammalian and microbial single-use stirred tank bioreactors}, journal = {Xcellerex Inc. (USA)}, year = {2010} }
Koch, S.	Cultivation of different eucaryotic cell types in single-use bioreactors – case studies with different products [Abstract]	2010	Probiogen AG (Germany)	standard
Abstract: The success of a new therapeutic product depends not only on its results in preclinical and clinical trials but to a great extent on the efficiency of process development and the cost-effectiveness of production processes as well as the timeto market. The disposable equipment allows for very short change-over procedures without the necessity of cleaning validation, it supersedes sterilization-in-place procedures and reduces the amount to be invested. The suitability of single-use bioreactors for the production of proteins and other products for therapeutic application is an often-discussed issue between product development companies, contract manufacturers and authorities. The presentation shows how the single-use bioreactor S.U.B. has been used successfully in fermentation processes with different eukaryotic cells for the production of a variety of products on different scales. Data characterizing robustness of the processes, predictability of the yield, product quality and scalability are discussed.
BibTeX: @standard{Koch2010, author = {Susann Koch}, title = {Cultivation of different eucaryotic cell types in single-use bioreactors – case studies with different products}, journal = {Probiogen AG (Germany)}, year = {2010} }

Created by JabRef on 23/03/2010.